Six Trichinella isolates (ISS03, ISS13, ISS10, ISS02, ISS029, ISS035) were detected by real-time PCR. CT values connected with melting curve analysis allowed a distinct species identification from the beginning of the reaction and HRM curve analysis showed individual melting curve for each of the studied species which can be used for genomic control on subsequent diagnostic tests. Obtained results determined the method as sensitive, accurate and appropriate for prevention of trichinellosis. The data of this study provide an additional and re-evaluate the existing information through which a new preventative and disease-fighting approach can be accomplished.
Key words: trichinellosis, real-time PCR
Topic: BIOLOGY