UHPLC-HRMS Based Saponin and Flavonoid Profiling of Chenopodium bonus-henricus L. Roots and Prolipase Activity of the Main Saponins

Abstract

UHPLC-HRMS profiling method was used for a comprehensive study of flavonoid and saponin-rich fractions from the MeOH extract of C. bonushenricus L. roots. Thirty-one compounds, respectively, 16 saponins of five sapogenins (oleanoic acid, 2-hydroxyoleanoic acid, medicagenic acid, bayogenin, and phytolaccagenin), together with 15 flavonoid glycosides of 6-methoxykaempferol, isorhamnetin, spinacetin, and patuletin were detected. Lipase modulatory activities of the main saponins were established by measuring the levels of the released 4-nitrophenol using LC-MS. All saponins exhibited prolipase activity and could find application in the treatment of cachexia.