Effects of Vitrification Temperatures and Concentration of Cryoprotective Agents on the Cytoskeleton in Bovine Immature Oocytes

Authors

  • Shiyu Zhao College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China
  • Jiaqi Liu College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China
  • Xiaojing Wang College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China
  • Yiheng Liu College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China
  • Xiaoxia Li College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China
  • Zhiqian Xu College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China
  • Yinghua Li College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China
  • Xueli Yu College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China

DOI:

https://doi.org/10.7546/CRABS.2023.04.18

Keywords:

oocyte, cytoskeleton, vitrification temperatures, concentration of cryoprotective agents

Abstract

The objective of the research was to evaluate the effect of vitrification temperatures (VTs) and concentration of cryoprotective agents (CPAs) on the cytoskeleton of bovine immature oocytes (BIOs). Bovine cumulus oocyte complexes (COCs) were collected and divided randomly into five groups: (a) fresh control group, (b) oocytes vitrified in liquid helium (LHe; −269◦C with 5.6 M CPAs, (c) oocytes vitrified in LHe (−269◦C with 6.6 M CPAs, (d) oocytes vitrified in liquid nitrogen (LN; −196 ◦C with 5.6 M CPAs, (e) oocytes vitrified in LN (−196◦C with 6.6 M CPAs. The results showed that the developmental level of BIOs in the LHe 5.6 M group was the highest among the four vitrification groups, and the maturation rate and cleavage rate were significantly higher than the other three vitrification groups (P < 0.05). With further experiments, the normal rate of microfilament and spindle in the LHe 5.6 M group was the highest compared with the other three vitrification groups. It was concluded that lower VT (−269◦C and lower CPAs (5.6 M) could significantly reduce damage to the cytoskeleton, which had a positive effect on the development of oocytes after vitrification.

Author Biographies

Shiyu Zhao, College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China

Mailing Address:
College of Animal Science and Technology,
Henan University of Science and Technology
Luoyang, Henan 471023, China
263 Kaiyuan St, Luoyang, China

and

Henan Provincial Key Laboratory
for Grass-Feeding Animals
Luoyang, 471023, China

E-mail: 1736427910@qq.com

Jiaqi Liu, College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China

Mailing Address:
College of Animal Science and Technology,
Henan University of Science and Technology
Luoyang, Henan 471023, China
263 Kaiyuan St, Luoyang, China

and

Henan Provincial Key Laboratory
for Grass-Feeding Animals
Luoyang, 471023, China

E-mail: 1808397962@qq.com

Xiaojing Wang, College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China

Mailing Address:
College of Animal Science and Technology,
Henan University of Science and Technology
Luoyang, Henan 471023, China
263 Kaiyuan St, Luoyang, China

and

Henan Provincial Key Laboratory
for Grass-Feeding Animals
Luoyang, 471023, China

E-mail: wxj15139918259@163.com

Yiheng Liu, College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China

Mailing Address:
College of Animal Science and Technology,
Henan University of Science and Technology
Luoyang, Henan 471023, China
263 Kaiyuan St, Luoyang, China

and

Henan Provincial Key Laboratory
for Grass-Feeding Animals
Luoyang, 471023, China

E-mail: liu15037826771@126.com

Xiaoxia Li, College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China

Mailing Address:
College of Animal Science and Technology,
Henan University of Science and Technology
Luoyang, Henan 471023, China
263 Kaiyuan St, Luoyang, China

and

Henan Provincial Key Laboratory
for Grass-Feeding Animals
Luoyang, 471023, China

E-mail: xxcphzs@126.com

Zhiqian Xu, College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China

Mailing Address:
College of Animal Science and Technology,
Henan University of Science and Technology
Luoyang, Henan 471023, China
263 Kaiyuan St, Luoyang, China

and

Henan Provincial Key Laboratory
for Grass-Feeding Animals
Luoyang, 471023, China

E-mail: 9906187@haust.edu.cn

Yinghua Li, College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China

Mailing Address:
College of Animal Science and Technology,
Henan University of Science and Technology
Luoyang, Henan 471023, China
263 Kaiyuan St, Luoyang, China

and

Henan Provincial Key Laboratory
for Grass-Feeding Animals
Luoyang, 471023, China

E-mail: Liyinghua2008@yeah.net

Xueli Yu, College of Animal Science and Technology, Henan University of Science and Technology and Henan Provincial Key Laboratory for Grass-Feeding Animals, China

Mailing Address:
College of Animal Science and Technology,
Henan University of Science and Technology
Luoyang, Henan 471023, China
263 Kaiyuan St, Luoyang, China

and

Henan Provincial Key Laboratory
for Grass-Feeding Animals
Luoyang, 471023, China

E-mail: yxl4282333@126.com

Downloads

Published

30-04-2023

How to Cite

[1]
S. Zhao, “Effects of Vitrification Temperatures and Concentration of Cryoprotective Agents on the Cytoskeleton in Bovine Immature Oocytes”, C. R. Acad. Bulg. Sci., vol. 76, no. 4, pp. 646–654, Apr. 2023.

Issue

Vol. 76 No. 4 (2023)

Section

Agricultural Sciences

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