In Vitro Cultivation of Saffron (Crocus sativus L.) and Assessment of Genetic Homogeneity Using iPBS Markers

Authors

  • Maria Petrova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Georgi Bonchev Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Lyudmila Dimitrova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Margarita Dimitrova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Roumiana Vassilevska-Ivanova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.7546/CRABS.2023.11.04.

Keywords:

corm, tissue culture, organogenesis, surface sterilization, molecular markers

Abstract

This research was an attempt to develop a protocol for the most optimal in vitro cultivation of saffron (Crocus sativus L.). The dried stigmas of the plant are known as spice imparting colour, flavour and aroma to foods and beverages. The appropriate disinfection procedure to introduce corm explants into in vitro culture was established. The optimum corm formation and corm growth (mean number of 3.6 per explant) was achieved on Murashige and Skoog (MS) medium containing 5 mg/l 6-benzylaminopurine (BAP) and 1 mg/l indole-3-acetic acid (IAA), while MS supplemented with 1 mg/l indole-3-butyric acid (IBA) was the best medium for root development. The fingerprinting profiles of the field saffron and the in vitro cultures assessed by iPBS markers were found identical which implies that no genetic alterations have occurred in response to in vitro cultivation. 

Author Biographies

Maria Petrova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Mailing Address:
Institute of Plant Physiology and Genetics,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: marry_petrova@yahoo.com

Georgi Bonchev, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Mailing Address:
Institute of Plant Physiology and Genetics,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: bonchevg@mail.bg

Lyudmila Dimitrova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Mailing Address:
Institute of Plant Physiology and Genetics,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: dim.lyudmila@gmail.com

Margarita Dimitrova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Mailing Address:
Institute of Plant Physiology and Genetics,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: mstoyadinova@abv.bg

Roumiana Vassilevska-Ivanova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Mailing Address:
Institute of Plant Physiology and Genetics,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 21
1113 Sofia, Bulgaria

E-mail: ru_vas_bg@yahoo.com

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Published

27-11-2023

How to Cite

[1]
M. Petrova, G. Bonchev, L. Dimitrova, M. Dimitrova, and R. Vassilevska-Ivanova, “In Vitro Cultivation of Saffron (Crocus sativus L.) and Assessment of Genetic Homogeneity Using iPBS Markers”, C. R. Acad. Bulg. Sci., vol. 76, no. 11, pp. 1670–1678, Nov. 2023.

Issue

Section

Biology