Anti-cancer Effect of Neocuproine on Oxidative Stress and Inflammatory Levels Induced by Neuroblastoma Cancer Cells

Authors

  • Filiz Demirdogen Department of Neurology, Mengücek Gazi Education and Research Hospital, Binali Yıldırım University, Turkey
  • Fatma Yesilyurt Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, Turkey
  • Aysenur Budak Savas Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, Turkey
  • Guven Akcay Department of Biophysics, Faculty of Medicine, Hitit University, Turkey
  • Ahmet Hacimuftuoglu Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, Turkey https://orcid.org/0000-0002-9658-3313

DOI:

https://doi.org/10.7546/CRABS.2024.04.14

Keywords:

inflammatory, neocuproine, neuroblastoma, oxidative stress, SH-SY5Y

Abstract

Neocuproine is a free radical scavenger. Neuroblastoma cancer is seen mostly in children. It accounts for approximately 15% of all pediatric cancer deaths. Treatments against SH-SY5Y may be ineffective due to the development of drug resistance, resulting in poor survival in high-risk patients. The goal of this study was to find out how the anti-cancer concentration of Neocuproine affected the levels of oxidants, antioxidants, and cytokines in the SH-SY5Y cell line. Five different concentrations of Neocuproine (12.5, 25, 50, 100, and 200 µM) were applied to the cell line for 24 h. MTT cytotoxicity tests, total oxidant status and total antioxidant capacity tests, and anti-inflammatory cytokine tests were used to look at the uses of Neocuproine. In our results, 100 µM Neocuproine application reduced cancer cell viability up to 69.76%. In addition, it was determined that 100 µM Neocuproine reduced oxidant activity, increased antioxidant capacity, and regulated anti-inflammatory cytokine levels in neuroblastoma cells, consistent with the cytotoxicity test. As a result, it was concluded that Neocuproine has an anti-cancer effect on the SH-SY5Y cell line.

Author Biographies

Filiz Demirdogen, Department of Neurology, Mengücek Gazi Education and Research Hospital, Binali Yıldırım University, Turkey

Mailing Address:
Department of Neurology,
Mengücek Gazi Education and Research Hospital,
Binali Yıldırım University,
Erzincan, Turkey

E-mail: fdemirdogen24@gmail.com

Fatma Yesilyurt, Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, Turkey

Mailing Address:
Department of Medical Pharmacology,
Faculty of Medicine,
Atatürk University,
Erzurum, Turkey

E-mail: fatmayesilyurttt@gmail.com

Aysenur Budak Savas, Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, Turkey

Mailing Address:
Department of Medical Pharmacology,
Faculty of Medicine,
Atatürk University,
Erzurum, Turkey

E-mail: aysenur.budak@ogr.atauni.edu.tr

Guven Akcay, Department of Biophysics, Faculty of Medicine, Hitit University, Turkey

Mailing Address:
Department of Biophysics,
Faculty of Medicine,
Hitit University,
Çorum, Turkey

E-mail: guvenakcay@hitit.edu.tr

Ahmet Hacimuftuoglu, Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, Turkey

Mailing Address:
Department of Medical Pharmacology,
Faculty of Medicine,
Atatürk University,
Erzurum, Turkey

E-mail: hacimuftuoglu@gmail.com

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Published

26-04-2024

How to Cite

[1]
F. Demirdogen, F. Yesilyurt, A. Budak, G. Akcay, and A. Hacimuftuoglu, “Anti-cancer Effect of Neocuproine on Oxidative Stress and Inflammatory Levels Induced by Neuroblastoma Cancer Cells”, C. R. Acad. Bulg. Sci., vol. 77, no. 4, pp. 584–591, Apr. 2024.

Issue

Section

Medicine