Antibacterial Properties of Wurtzite-hydrozincite Mixed-phase Phyto-synthesized ZnO Particles

Authors

  • Rumyana Eneva Department of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences
  • Daniela Stoyanova Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences
  • Irina Stambolova Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences
  • Simona Mitova Department of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences
  • Stephan Engibarov Department of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences
  • Irina Lazarkevich Department of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.7546/CRABS.2024.06.04

Keywords:

green synthesis, wurtzite-hydrozincite nanoparticles, antibacterial activity, Aeromonas caviae, Erysipelothrix rhusiopathiae

Abstract

In this study, wurtzite-hydrozincite mixed-phase nanoparticles were prepared using a Mentha arvensis leaves extract as a stabilizing/reducing agent, followed by hydrothermal treatment at 180 °C. The resulting powder samples were characterized by X-ray diffraction (XRD) phase analysis and by Transmission Electron Microscopy (TEM). The existence of biphasic zinc oxide nanoparticles with hydrozincite and wurtzite crystallographic phases was proven. The particles sizes of biosynthesized oxide powders decreased upon the increase in the Mentha arvensis extract concentration. The antibacterial properties of the nanoparticles were investigated under dynamic contact conditions and the resazurin microplate method, using test strains with diverse structural and biochemical features. The effect of mixed-phase ZnO nanoparticles on the animal pathogens Erysipelothrix rhusiopathiae and Aeromonas caviae is studied for the first time. It was established, that the three powders exert significant antibacterial activity, which is higher against Gram-negative test microorganisms, whereupon the sample with the smallest particle sizes (G-Z3) exhibits the most effective inhibition. It could be supposed that the combination of factors: the presence of two phases, reactive oxygen species formation; small particles sizes and surface defects have beneficial effect on enhancement of their antibacterial properties.

Author Biographies

Rumyana Eneva, Department of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences

Mailing Address:
Department of General Microbiology,
The Stephan Angeloff Institute of Microbiology,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 26
1113 Sofia, Bulgaria

Email: rum_eneva@abv.bg

Daniela Stoyanova, Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences

Mailing Address:
Department of Surface Chemistry,
Laboratory of Materials and Processes in Environmental Protection,
Institute of General and Inorganic Chemistry,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 11
1113 Sofia, Bulgaria

Email: dsto@svr.igic.bas.bg

Irina Stambolova, Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences

Mailing Address:
Department of Surface Chemistry,
Laboratory of Materials and Processes in Environmental Protection,
Institute of General and Inorganic Chemistry,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 11
1113 Sofia, Bulgaria

Email: stambolova@yahoo.com

Simona Mitova, Department of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences

Mailing Address:
Department of General Microbiology,
The Stephan Angeloff Institute of Microbiology,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 26
1113 Sofia, Bulgaria

Email: simona.mitova9@abv.bg

Stephan Engibarov, Department of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences

Mailing Address:
Department of General Microbiology,
The Stephan Angeloff Institute of Microbiology,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 26
1113 Sofia, Bulgaria

Email: stefan_engibarov@abv.bg

Irina Lazarkevich, Department of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences

Mailing Address:
Department of General Microbiology,
The Stephan Angeloff Institute of Microbiology,
Bulgarian Academy of Sciences
Akad. G. Bonchev St, Bl. 26
1113 Sofia, Bulgaria

Email: irinalazarkevich@abv.bg

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Published

30-06-2024

How to Cite

[1]
R. Eneva, D. Stoyanova, I. Stambolova, S. Mitova, S. Engibarov, and I. Lazarkevich, “Antibacterial Properties of Wurtzite-hydrozincite Mixed-phase Phyto-synthesized ZnO Particles”, C. R. Acad. Bulg. Sci. , vol. 77, no. 6, pp. 817–823, Jun. 2024.

Issue

Section

Biology